Cover crops can shelter competent squash phyllosphere bacteria to reduce Pseudomonas syringae symptoms

Speed talk
Part of:
11:20, Friday 20 Dec 2019 (5 minutes)
Room EFG

Pseudomonas syringae, is considered one of the most important bacterial plant pathogens. Soil conservation practices, such as cover crops, are amongst tools that can contribute to reducing disease pressure caused by bacteria. We have recently demonstrated that leaf surface (phyllosphere) bacterial community structure changed when squash is grown with a rye cover crop treatment, followed by a decrease of Pseudomonas syringae symptoms. In this work, our goal was to describe the differential bacterial abundance of the squash phyllosphere. We pursue the hypothesis that some phyllosphere bacteria could decrease the P.syringae symptoms. Thus, we grew squash, within a two-year field, in four agricultural practices: bare soil, cover crops, chemically terminated cover crops and plastic cover. We sampled squash leaves at 3 different dates. From samples wash a bacterial bank was built with cultivable strains. Each isolated strain where identify by 16S sequencing and subjected to a competition assay on Petri dish against P.syringae. More than thirty strains, belonging to the genus Acinetobacter, Rhizobium, Delftia, Pseudomonas among others, were proven to inhibits P.syringae growth on media. In our current work we are conducting a competition assay in a greenhouse with 20 of the best P.syringae antagonists. First trial already shown promising defense response against this pathogen from several selected bacterial candidate, originally isolated from cover crops treatment.

Ph D Candidat

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