The wild blueberry rhizosphere microbiome

Poster session
Partie de:
11:30, vendredi 20 déc. 2019 (1 heure)
Dîner   12:30 PM à 01:30 PM (1 heure)
Salle ABCD

Canada is the largest producer of wild blueberries in the world. The crops are grown in boreal regions using two species belonging to the Ericaceae family: Vaccinium angustifolium and Vaccinium myrtilloides. The main edaphic properties of ericaceous culture are high carbon to nitrogen ratio and low pH. The contribution of ericoid mycorrhizal fungi is essential as they increase the nitrogen and phosphate supply, by accessing non-assimilable sources by the plants. Field studies on microbial diversity associated with wild blueberry roots, especially ericoid mycorrhizal fungi, remain fragmentary. This part of the project aimed to characterize the fungal and bacterial communities of the wild blueberry rhizosphere. Nine plots were selected, with the advice of the blueberry producer within three commercial blueberry fields in the Lac St-Jean region of Quebec. The sampling consisted of extracting blueberry shrubs including their shoots, roots and adhering soil. Five rhizospheric soil samples of V. angustifolium were collected per plots, for a total of 45 samples. We proceeded to the DNA extraction and amplification of 16S and ITS regions. Sequencing was done using Illumina MiSeq and DADA2 was used for bio-informatics analyses. The results show a greater variability in terms of structure in the fungal community than in the bacterial community. Rhizobiales (containing nitrogen fixing bacteria) and Helotiales (containing ericoid mycorrhiza fungi) are the most abundant orders which suggest a certain importance for the blueberry plants. The microbial communities also vary according to the establishment date of the fields.


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