The rhizosphere metagenomes of ancestral and modern wheat cultivars grown under low fertiliser inputs are not significantly different

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vendredi 20 déc. 11:30 AM (1 heure)
Dîner   12:30 PM à 01:30 PM (1 heure)
Salle ABCD

With the rock phosphate reserves approaching depletion and the increasing concerns about the environmental and economic costs of fertilizer use in agriculture, there is mounting pressure to reduce their intensive use. However, recent breeding efforts were mostly carried out under high levels of fertilizers, which has led to the hypothesis that modern wheat cultivars might have lost their capacity to associate with soil microbes that would help them acquire nutrients from the soil under low fertilization conditions. To test this hypothesis, ten ancestral and modern wheat cultivars belonging to two wheat species (Triticum aestivum-hexaploid, Triticum turgidum-tetraploid) were seeded in a field experiment under low fertilization conditions. At the post-anthesis stage, the rhizosphere soil was collected, its DNA extracted and sent for shotgun metagenomic sequencing. In contrast to our hypothesis, there was no significant difference in the rhizosphere metagenomes of the different cultivars, and this held true when focusing the analyses on specific taxonomic or functional categories of genes. The main factor influencing the rhizosphere metagenome was space, with samples collected from different parts of the field showing significant differences in their genetic and taxonomic content. This variability was also visible for the yields and other agronomic parameters measured at the end of the growing season, which results in significant correlations between these parameters and N and P cycling genes, which would need to be further scrutinized to ascertain their roles in wheat nutrition and growth. Our study showed for the first time that the rhizosphere metagenome of wheat is stable across a wide variety of genotypes, which suggests that modern genotypes did not lose the ability to associate with beneficial nutrient-cycling soil microbes.

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