Comparing methods to assess the biodiversity of soil microbes

Poster session
Partie de:
11:30, vendredi 20 déc. 2019 (1 heure)
Dîner   12:30 PM à 01:30 PM (1 heure)
Salle ABCD
Plant productivity and community composition vary according to the microbial biodiversity in the soil. In fact, there is growing evidence that plants actively recruit certain microbes from the surrounding soil to assist their hosts in growth, productivity, and stress tolerance. In order to transition toward more sustainable agricultural systems, understanding the taxonomic structure and functions of these soil, and root, microbiomes would be a significant development. Therefore, microbial biodiversity in agriculture should be monitored and used to generate interesting hypotheses about how soil, and root, microbiomes impact the host plant, as well as how current farming practices impact the microbiomes. As part of my PhD project, I have generated 16S amplicons to catalogue the bacterial microbiomes of both the root, and its surrounding soil, from five Brassicaceae oil-seed crops grown under field conditions in Swift Current, Saskatchewan. Sixty plots were randomly planted in 2016 and 2017 following a randomized four block design with three treatments, which were the previous year’s crop of either wheat, lentils, or fallow. Samples were harvested at the full-flowering stage, split into root and soil compartments, and their total DNA extracted. A commercially available bacterial mock community was added to the samples as a positive control, prior to generating the 16S amplicons. Sequencing was done at Génome Québec using the Illumina MiSeq platform, followed by analysis with DADA2. Here, I will briefly discuss the results from this biodiversity survey of the soil, and root, microbiomes of five different Brassicaceae crops.

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