The Effect of the Synthetic Material in contact with DMEK Tissue During Staining on Endothelial Cell Loss

Paper Presentation | Présentation d'article
1:40 PM, vendredi 10 juin 2022 (5 minutes)

Authors: Abdullah Al-Kaabi1, Britta Gustavson1, Julia Talajic2, Johanna Choremis1
1McGill University, 2Université de Montréal.

Author Disclosure Block: A. Al-Kaabi: None. B. Gustavson: None. J. Talajic: None. J. Choremis: None.


Title: The Effect of the Synthetic Material in contact with DMEK Tissue During Staining on Endothelial Cell Loss


Abstract Body:

Purpose: Endothelial cell loss (ECL) during corneal graft preparation affects graft survival and the re-grafting rates.1-3During Descemet membrane endothelial keratoplasty (DMEK) donor tissue is incubated in Trypan blue ophthalmic solution, typically in either a glass or plastic vessel. DMEK grafts are exquisitely sensitive, with ECL documented from any form of touch.4 Furthermore, cellular damage of cells due to oxidative stress and mitochondrial depolarization secondary to exposure to micro-plastic chemicals has been established in the literature. 5-7 This study aimed to investigate how the material in contact with DMEK tissue during staining impacts the rate of ECL to better inform surgeons and material scientists when choosing materials for DMEK.
Study Design: Retrospective study conducted at Hôpital Maisonneuve-Rosemont (HMR) and Jewish General Hospital(JGH), Montréal, Canada.
Methods: Data were collected from all patients who underwent DMEK surgery by the same surgeon (Dr.J. Choremis) between January 2019 and June 2020 at two sites. Patients operated at JGH constituted the plastic group whereas patients operated at HMR were the glass group. Patients with ocular co-morbidities and patients who passed away within 6 months of surgery were excluded from all analyses. The primary endpoints were the rate of ECL, absolute ECL, and percentage ECL, at 12-30 months. The secondary endpoints were re-bubbling and graft failure rates, at ≥6 months. ECL was the difference between pre and post-operative specular microscopy counts. The unpaired student’s t-test, chi-squared test, and ANCOVA were used with a power of 0.8 and a significance of 0.05.
Results: The results demonstrate that there is a significant difference between plastic and glass groups in the rate of ECL at 12-30 months post-DMEK, with a smaller rate of loss in the plastic group (2.49% vs. 3.37% ECL/month, p=0.010). Future data beyond 30 months will help determine if these differences have clinically meaningful effect for patients and the need for re-grafting. There were no significant differences in the other endpoints and no confounding variables. In addition, there were no significant differences between the rates of re-bubbling or of graft failure at 6-30 months.
Conclusions: These data demonstrate that the choice of material used as a staining vessel for DMEK has a significant impact on the rate of ECL. This has practical implications for the choice of vessel and the development of new technologies for DMEK surgeries. Of note, the target sample size was not met therefore the power of these conclusions may be reduced. Nevertheless, these data support further investigation into the effect of synthetic materials on ECL in order to optimize surgical procedures to provide patients with the best chance for a successful DMEK surgery.

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