One size does not fit all, and while we embrace the world of distributed barcoding, the need to produce comprehensive and accurate reference libraries remains the foundation of DNA barcoding. Indeed, high-throughput sequencing has made barcode data generation accessible to research labs of all sizes. Yet the process of collecting, accessioning, reformatting samples, preparing DNA extracts, and creating multiplex PCR libraries for barcoding or metabarcoding remains an intricate web of decisions constantly challenged by numerous bottlenecks and barriers. As the volume of samples moving daily through each analytical step increases to thousands or tens of thousands, the risk of sample losses or other errors compromising data integrity rises. Core facilities, known for higher operational standards, excel in managing large sample aggregates and provide access to advanced analytical capacity beyond the reach of most research labs. This session will showcase expertise from core facilities specializing in biodiversity genomics research. It will cover unique approaches that employ diverse sequencing platforms but adhere to shared best practices and community standards. Presentations will deliver an overview of high-capacity barcoding workflows, the latest advances in the recovery of genomic information from organisms with low and poor DNA yields, sustainability efforts, and other insights into building next-generation labs of the future.
Agenda
DNA Barcoding à la carte: Blending sequencing and liquid handling technologies for sustainable workflow and affordable results.
Presenter: Evgeny Zakharov
Affiliation: Centre for Biodiversity Genomics, University of Guelph
Format: Full Talk
A genomics hub in the Amazon: decolonizing the generation, analyzes, and use of genomic data.
Presenter: Alexandre Aleixo
Affiliation: Instituto Tecnológico Vale - DS
Format: Full Talk
Towards large-scale DNA species identification methods from fresh sampling to museum collection mining.
Presenter: Elza Duijm
Affiliation: Naturalis Biodiversity Center
Format: Full Talk
Distributed barcoding using a simple and cost-effective end-to-end solution.
Presenter: Sean Prosser
Affiliation: Centre for Biodiversity Genomics, University of Guelph
Format: Full Talk